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IntroductionBrief description of system
EcoR124I
is a Type I restriction modification system. It is a protein complex made up
of a core Methyltransferase (MTase) consisting of 1 HsdS (Specificity)
subunit and 2 HsdM (Methylase) subunits. A first then a second HsdR subunit
bind to the MTase to form a restriction deficient R1-complex
and then a restriction-proficient Endonuclease (ENase) complex
Type
I R-M enzymes,
as either a R1 or
R2 complex,
translocate the DNA in the presence of ATP and Mg2+.
EcoKI is the most studied Type I R-M system and all models have until now been
based upon this enzyme.
In the first 3 months of my studies data has been collected which is
beginning to show significant differences between EcoKI and EcoR124I.
The primary difference noted so far is the dimerisation undertaken by
the EcoKI system prior to translocation and cleavage
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