PAPERS READ AND POSTERS PRESENTED

Glover, S.W. & Firman, K. Control of plasmid-coded restriction systems. SOCIETY FOR GENERAL MICROBIOLOGY SYMPOSIUM, LEEDS, 1980.

Firman, K. The switch in specificity of the bacterial restriction and modification system EcoR124. NORTHERN BACTERIAL GENETICS SYMPOSIUM, LEEDS, 1980.

Firman, K. Interactions between the plasmids R124 and F. SOCIETY FOR GENERAL MICROBIOLOGY SYMPOSIUM, CAMBRIDGE, 1982.

Firman, K. The switch of the specificity of the R124 restriction and modification system. SGM - GENETICAL SOCIETY JOINT SYMPOSIUM UMIST, 1983.

Firman, K. & Glover, S.W. The alternate expression of restriction and modification specificity. XV INTERNATIONAL CONGRESS OF GENETICS, NEW DELHI, 1983.

Firman, K. & Glover, S.W. DNA rearrangements between the plasmids F and R124, XV INTERNATIONAL CONGRESS OF GENETICS, NEW DELHI, 1983.

Firman, K. & Glover, S.W. The cloned R124 and R124/3 restriction and modification genes. SOCIETY FOR GENERAL MICROBIOLOGY SYMPOSIUM, BIRMINGHAM, 1984.

Firman, K. & Glover, S.W. The switch in specificity of the restriction and modification system EcoR124. SOCIETY FOR GENERAL MICROBIOLOGY SYMPOSIUM, BIRMINGHAM, 1984.

Firman, K. & Glover, S.W. DNA rearrangements associated with F-like origins. INTERNATIONAL CONGRESS OF MICROBIOLOGY, MANCHESTER, 1986.

Firman, K. & Glover, S.W. Two closely related type I restriction and modification systems - EcoR124 and EcoR124/3. INTERNATIONAL CONGRESS OF MICROBIOLOGY, MANCHESTER, 1986.

Firman, K Evolution of type I restriction and modification specificity. SOCIETY FOR GENERAL MICROBIOLOGY SYMPOSIUM, DURHAM, 1987.

Firman, K. Precise excision of the gamma-delta transposon. SOCIETY FOR GENERAL MICROBIOLOGY SYMPOSIUM, WARWICK, 1988.

Dutta, C., Patel, J., Taylor, I & Firman, K. DNA recognition by type I restriction endonucleases. PROTEIN ENGINEERING - GRONIGEN, 1989.

Taylor, I., Patel, J., Firman, K. & Kneale, G.G. Purification and renaturation studies of the DNA recognition subunit of EcoR124. BRITISH BIOPHYSICAL SOCIETY MEETING, NEWCASTLE-UPON-TYNE (1990).

Abadjieva, A., Firman, K., Glover, S.W., Hubacek, J. & Weiserova, M. A new class of mutations in the hsdM gene for type I restriction enzymes. Molecular Genetics of Bacteria & Phages, COLD SPRING HARBOR LABORATORIES, COLD SPRING HARBOR, NEW YORK, (1992).

Zinkevich, V., Glover, S.W., Firman, K., Hubacek, J. & Weiserova, M. Mutations in hsdS that affect assembly of type I restriction enzymes. Molecular Genetics of Bacteria & Phages, COLD SPRING HARBOR LABORATORIES, COLD SPRING HARBOR, NEW YORK, (1992).

Patel, J. & Firman, K. The domain structure and DNA binding properties of type I restriction and modification systems (lecture). 11th EUROPEAN MEETING ON GENETIC TRANSFORMATION, BUDAPEST (1992).

Abadjieva, A., Zinkevich, V., Weiserova, M., Patel, J., & Firman, K. The domain structure and subunit assembly of type I restriction and modification systems (poster). 11th EUROPEAN MEETING ON GENETIC TRANSFORMATION, BUDAPEST (1992).

Abadjieva, A., Patel, J. & Firman, K. Mutations in the DNA binding subunit of EcoR124 that produce a novel DNA specificity. 11th INTERNATIONAL BIOPHYSICS CONGRESS, BUDAPEST (1993a).

Abadjieva, A., Patel, J., Webb, M. & Firman, K. (1993b) Deletion mutants of the EcoR124 restriction-modification system with novel DNA specificity. RESTRICTION ENDONUCLEASES AND MODIFICATION METHYLTRANSFERASES STRUCTURES AND MECHANISMS, FASEB, VERMONT, USA

Firman, K., Development of a hypertext teaching system based on the ToolBook authoring software, in ASSOCIATION FOR EDUCATIONAL AND TRAINING TECHNOLOGIES. 1994: NAPIER UNIVERSITY, EDINBURGH.

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Abadjieva, A. Patel, J., Weiserova, M., Janscak, P., Hubacek, J., Zinkevich, V., Dutta, C., and Firman, K. Analysis of the domain organisation of the DNA binding subunit of type I restriction endonucleases.  7th INTERNATIONAL CONGRESS OF BACTERIOLOGY AND APPLIED MICROBIOLOGY, IUMS, PRAGUE (1994).

Zinkevich, V., Weiserova, M. Janscak, P., Abadjieva, A., Kryukov, V. and Firman, K. Temperature-sensitive mutations within the HsdS and HsdM genes of EcoKI which appear to affect subunit-subunit interactions. 7th INTERNATIONAL CONGRESS OF BACTERIOLOGY AND APPLIED MICROBIOLOGY, IUMS, PRAGUE (1994).

Abadjieva, A., Webb, M., Patel, J., Zinkevich, V. and Firman, K. Deletions within the DNA recognition subunit of M.EcoR124I that identify a region involved in protein‑protein interactions between HsdS and HsdM. 7th INTERNATIONAL CONGRESS OF BACTERIOLOGY AND APPLIED MICROBIOLOGY, IUMS, PRAGUE (1994).

Holubova, I., Janscak, P., Weiserova, M., Benada, O., Hubacek, J. and Firman, K. Cellular localisation of the type I restriction and modification system of Escherichia coli K-12. 7th INTERNATIONAL CONGRESS OF BACTERIOLOGY AND APPLIED MICROBIOLOGY, IUMS, PRAGUE (1994).

Janscak, P., Weiserova, M., Benada, O., Hubacek, J. and Firman, K. Substrate preference of R.EcoKI restriction endonuclease. 7th INTERNATIONAL CONGRESS OF BACTERIOLOGY AND APPLIED MICROBIOLOGY, IUMS, PRAGUE (1994).

Janscak, P. and Firman, K. Structure-function differences among type I restriction endonucleases.  MOLECULAR GENETICS OF BACTERIA AND PHAGES, COLD SPRING HARBOR LABORATORIES, USA (1995)

Janscak, P. and Firman, K.  DNA binding and cleavage by the type IC restriction endonuclease EcoR124I. MOLECULAR GENETICS OF BACTERIA AND PHAGES, COLD SPRING HARBOR LABORATORIES, USA (1995)

Janscak, P. & Firman, K. The influence of quaternary structure on the function of the type IC restriction endonuclease EcoR124I. 4TH NEW ENGLAND BIOLABS WORKSHOP ON BIOLOGICAL DNA MODIFICATION, IGLS, INNSBRUCK, AUSTRIA (1997).

Weiserova, M. & Firman, K. (1997). Point mutations in the central conserved region of the DNA recognition subunit of R.EcoR124I affecting subunit assembly of the endonuclease. 4TH NEW ENGLAND BIOLABS WORKSHOP ON BIOLOGICAL DNA MODIFICATION, IGLS, INNSBRUCK, AUSTRIA.

Akimkina, T., Kostrov, S., Ivanov, P., Bonch-Osmolovskaya, Sokolova, T., Firman, K. and McClellan, J. (1998).  A novel plasmid fgrom the extreme thermophile Thermotoga maritima MC24 is negatively supercoiled.  THERMOPHILES 98, BREST, FRANCE.

Slater, J. L., Mitchell, J. I., Firman, K., Bremer, G. B., Lambert, C. & Duchars, M. (1999). A molecular study of N-3 polyunsaturated fatty acid production by the marine fungoid protist Thraustochytrium striatum Schneider. Marine Mycology Symposium, HONG KONG.

Keith Firman and Mark Szczelkun (1999).  EcoR124I restriction endonuclease as a molecular motor.  144^th Ordinary Meeting of the SGM – Symposium Molecular Machines: Mobile protein complexes in micro-organisms, LEEDS, UK.

Keith Firman, Mark Szczelkun and Christina F Dutta (2001). Type I restriction-modification enzymes: DNA-based molecular motors.  ACS Prospectives Conference Series.  Biological Applications of Nanotechnology.  BERKLEY, CALIFORNIA USA.  June 3-6.

Keith Firman, Mark Szczelkun and Christina F Dutta (2001). Type I restriction-modification enzymes: DNA-based molecular motors.  BBSRC Workshop, Molecular Motors: Biology, Biophysics and applications.  SCARMAN HOUSE, UNIVERSITY WARWICK.

Keith Firman (2001). A DNA-based molecular motor capable of acting as an actuator in nanotechnological devices.  Southern Bioscience Bring and Bio – TECHNOLOGY TRANSFER FAIR, UNIVERSITY OF READING.

Keith Firman (2002). Targeted Nano-Solutions in Healthcare.  BioNanoTech Conferences to collate Expressions of Interest for 6^th Framework Networks of Excellence.  MÜNSTER, GERMANY.

Alex Blundell, John van Noort, Keith Firman (2003). Structural Studies of a DNA-Binding Molecular Motor.  HOUSES OF PARLIAMENT, LONDON

Ralf Seidel, Carsten van der Scheer, John van Noort, Keith Firman, Cees Dekker (2003).  Translocation of type I restriction endonuclease EcoR124I along DNA measured with magnetic tweezers.  BIOPHYSICAL SOCIETY, BALTIMORE, USA.

Marie Weiserová, Štěpánka Vejsadová, Keith Firman (2003).  Type I Restriction-Modification Enzymes as Molecular Motors in Bio-Nanotechnology, Nanosciences, nanotechnologies and nanomaterials, Czech Society for New Materials and Technologies, Prague and Faculty of Mechanical Engineering, BRNO UNIVERSITY OF TECHNOLOGY, CZECH REPUBLIC.

Alex Blundell, John van Noort and Keith Firman (2003).  Structural studies of a DNA-binding molecular motor.  Nanoforum Workshop on Nanomaterials and Applications, SINAIA, ROMANIA.

Keith Firman (2003).  Linking the biological and silicon worlds through the use of a molecular motor.  2^nd Defence Nanotechnology Conference, MILLENIUM GLOUCESTER HOTEL, LONDON.

J. van Noort, R. Seidel, Cees Dekker, C.F. Dutta,  T. Robinson and K. Firman (2004).   A Molecular Motor that Links the Biological and Silicon Worlds.  NanoTech 2004, BOSTON, USA.

Olga Kazakova, Lizzie Brown, Catherine Halliwell, John Gallop, Keith Firman, and Christina Dutta (2004). Nanomagnetic studies of molecular motors.  Condensed Matter and Materials Physics Conference (CMMP04), UNIVERSITY of WARWICK, UK.

Keith Firman (2004).  Molecular Motors. FET Information Day, BRUSSELS, BELGIUM.

Terry Robinson, Steven Magennis, David Dryden and Keith Firman (2004).  Fluorescence Studies of the EcoR124I Molecular Motor.  5^th NEB meeting on restriction/modification, Bristol UNIVERSITY OF BRISTOL, UK.

Alex Blundell, Christina Dutta, Terry Robinson, Marie Weiserova and Keith Firman (2004).  Structure-Function of the EcoR124I Restriction-Modification Enzyme.  5^th NEB meeting on restriction/modification, Bristol UNIVERSITY OF BRISTOL, UK

Peter Coxhead, Christina Dutta, James Smith, Olga Kazakova, John van Noort, Thijn van der Heijden, Cees Dekker and Keith Firman (2004).  AFM analysis of DNA Translocation by EcoR124I.  5^th NEB meeting on restriction/modification, Bristol UNIVERSITY OF BRISTOL, UK.

Terry Robinson, Olga Kazakova, Catherine Halliwell, Christina Dutta, John Gallop and Keith Firman (2004).  Surface attachment and Self-Assembly of a Molecular Motor.  5^th NEB meeting on restriction/modification, Bristol UNIVERSITY OF BRISTOL, UK.

Keith Firman, John Gallop, Cees Dekker, David Bensimon, Roberto Favilla and Marie Weiserova (2004).  Mol Switch: an EU-funded project.  5^th NEB meeting on restriction/modification, Bristol UNIVERSITY OF BRISTOL, UK.

I. Holubová, Š. Vejsadová, K. Firman, M. Weiserová (2004).  Family dependent variations in membrane topology of the Type I  Restriction-Modification enzymes.  5^th NEB meeting on restriction/modification, Bristol UNIVERSITY OF BRISTOL, UK.

Ralf Seidel, John van Noort, Carsten van der Scheer, Joost Bloom, Nynke Dekker, Terence Robinson, Christina F. Dutta, Alex Blundell, Keith Firman, and Cees Dekker (2004).  Translocation of Type I restriction endonuclease EcoR124I along DNA measured with magnetic tweezers.  5^th NEB meeting on restriction/modification, Bristol UNIVERSITY OF BRISTOL, UK.

Eva Šišáková, Keith Firman, Marie Weiserová (2004).  Mutagenic analysis of the endonuclease catalytic motif of the HsdR subunit of Type IC enzyme EcoR124I.  5^th NEB meeting on restriction/modification, Bristol UNIVERSITY OF BRISTOL, UK.

Štěpánka Vejsadová, Keith Firman, Marie Weiserová (2004).  Subunit Assembly Mutants of the Type I R-M enzyme EcoR124I.  5^th NEB meeting on restriction/modification, Bristol UNIVERSITY OF BRISTOL, UK.

Keith Firman (invited) (2004).  A Biological Molecular Motor with the potential to be a self-assembling nanoactuator.  3^rd International Conference NANO 04 BRNO, CZECH REPUBLIC.

Keith Firman (invited) (2004).  Bionanotechnology: motoring toward a nanoactuator.  Microtechnologies and Nanotechnologies Conference (Science and Technology week of the Basque Country), DONOSTIA/SAN SEBASTIAN, SPAIN.

Keith Firman (invited) (2005).  Mol Switch: A biological molecular motor with the potential to be a self-assembling nanoactuator.  NID Conference, Phantoms Network, MADRID, SPAIN.

Keith Firman (invited) (2005).  Biological Molecular Motors - toward a nanoactuator/biosensor device.  Annual IMM Symposium, NIJMEGEN, THE NETHERLANDS.

Ralf Seidel, Joost G. P. Bloom, Carsten van der Scheer, John van Noort, Nynke H. Dekker, Keith Firman_, Mark D. Szczelkun and Cees Dekker (2005).  Tracking of Type I restriction enzymes along the DNA.  Helicases and NTP driven nucleic acid machines, AROLLA, SWITZERLAND.

Keith Firman (invited) (2006).  Developing Molecular Motors for Nanotechnology.  FET Conference Group, BRUSSELS, BELGIUM.

Keith Firman (invited) (2006).  Building a nanoactuator: a biological molecular motor that can manipulate objects on the microscale.  ICT for Bio-Medical sciences and Healthcare, BRUSSELS, BELGIUM.

Keith Firman, Peter Coxhead, James Youell, John van Noort, Ralf Seidel and Cees Dekker (2006).  Single molecule analysis of DNA translocation by the molecular motor R.EcoR124I.  159^th Meeting of the SGM, University of YORK, UK.

Keith Firman (2007). Use of a molecular motor for reporting events at the single molecule level – a nanoactuator.  Nanoscale Physics and Technology: the Interface with Medical and Biological Sciences, Institute of Physics, University of SOUTHAMPTON, UK.

Keith Firman, Kathy McMahon and Ivo Utke (2007).  Attended a Workshop on Synthetic Biology at the EMERGENCE/TESSY Workshop, ZURICH, SWITZERLAND.

James Youell, Peter Coxhead and Keith Firman (2007).  Toward single-molecule sensing: a nanoactuator that reports biological events at the single-molecule level.  NANO-EXPO, NOTTINGHAM, UK.

James Youell, Peter Coxhead and Keith Firman (2007).  Toward single-molecule sensing: a nanoactuator that reports biological events at the single-molecule level. 3rd International Conference on Synthetic Biology (Synthetic Biology 3.0), ZURICH, SWITZERLAND.

Additional (recent) Conferences attended:                

SMi Conference on High-throughput screening of drugs, Hatton Gardens, London, May 2001.

Institute of Nanotechnology “Nanotechnology for Business”, Royal Society, May 2001.

LTSN Workshop on e-learning techniques, Bristol University, September 2001.

IUMS Conference, Paris, July 2002.

3i Conference on Nanotechnology, 3i Headquarters, London, July, 2002.

6^th Framework Training Conference by UKRO, National History Museum, November 2002.

DTI Workshop on 6^th Framework NoE bids, Rubens Hotel, London, January 2003.

Preparation of NanoMot Network – 6^th Framework Network bid, Dresden, February 2003.

NPL/DTI Mission Statement on BioNanotechnology, Royal Society, February 2003.

Functional Genomics, European Science Foundation, Prague May 2003.

EPSRC Media Training for Nanotechnology Researchers, British Philosophical Society, London August 2003.

Royal Society of Chemistry Bio-Nanotechnology Conference, Institute of Physics, London November 2003.

MNT Club Meeting, “The Measurement and Characterisation of Medical Biosensors”, NPL, Teddington, London.

In addition during 2001-2006:

I have prepared a series of posters on the subject of the Human Genome Sequencing Project for Littlehampton Museum.

Presented two seminars on Animal Cloning to St. Vincent’s 6^th Form College, Gosport.

Presented a series of seminars on Molecular Motors to The National Physics Labs., Delft University of Technology and BTG International Ltd.

I have chaired two Workshops of the BTG Nanotechnology Network (Appendix 2).

I have presented a seminar on Biological Molecular Motors to a group of 500+ 6^th-form students, at Fernham Hall, Fareham, for the Institute of Biology.

I chaired the 1^st Workshop of the EU-funded project Mol Switch at the Holiday Inn, Portsmouth.

The 3^rd NanoNet Workshop – Nanoparticles and Nanodevices for Biological Chemistry – held at the IBIS Hotel, Portsmouth.

The 4^th NanoNet Workshop – Force Microscopy for Biological Systems – held at the National Physical Laboratory, Teddington, UK.

Invited to the UK Embassy in Boston, USA and the Canadian High Commission, London to Network on nanotechnology.

Given two lectures to a school in Poole – “What is nanotechnology” and “Biological Molecular Motors”.

Partook in an event in Explore@Bristol about communication in science which involved interaction with young people in an open forum to discuss bionanotechnology.

Attended the Descartes Prize Awards, 2005, at the Royal Society, London.

Poster from the European Nanosciences Forum, 2006, was also presented at the European Parliament.

Invited to partake in a Foresight Institute Technology Roadmap Group Meeting (December 2006) in the USA (personal invite from Eric Drexler).          
Invited as a Road‑mapping Committee member to presentation of above roadmap at the Productive Nanosystems Conference, (October 2007), Washington DC, USA.

Invited to attend the Bio-Nano Interface meetings of the British Standards Institute.

Invited to partake in a MOD roadmap exercise at Porton Down (March 2006).

Invited as a road-mapping committee member to TESSY – Towards a European Strategy for Synthetic Biology, Zurich (June 2007).

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Author Dr Keith Firman,
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