Glover, S. W. & Firman, K. (1982). Genetic control of DNA specificity. In Genetic exchange (Streips, U. N., Goodgal, S. H., Guild, W. R. & Wilson, G. A., eds.), pp. 331-338. Marcel Dekker, New York.
Firman, K. (1982). The restriction and modification system of the bacterial plasmid R124. PhD, Newcastle upon Tyne.
Glover, S. W., Firman, K., Watson, G., Price, C. & Donaldson, S. (1983). The alternate expression of two restriction and modification systems. Molecular and General Genetics 190, 65-69.
Firman, K., Creasey, W. A., Watson, G., Price, C. & Glover, S. W. (1983). Genetic and physical studies of restriction-deficient mutants of the IncFIV plasmids R124 and R124/3. Molecular and General Genetics 191, 145-153.
Harper, S. H., Firman, K. & Glover, S. W. (1984). The interaction of mini-F with the plasmids R124 and R124/3. In Plasmids in bacteria (Helinski, D. R., Cohen, S. N., Clewell, D. B., Jackson, D. A. & Hollaender, A., eds.), pp. G20. Plenum Press, New York.
Firman, K., Price, C. & Glover, S. W. (1985). The EcoR124 and EcoR124/3 restriction and modification systems: cloning the genes. Plasmid 14, 224-234.
Price, C., Bickle, T. A., Firman, K. & Glover, S. W. (1989). Unequal crossing over as a mechanism to generate changes in DNA sequence recognition by the type I restriction and modification enzymes. Journal of Molecular Biology 205, 115-125.
Firman, K. (1991). DNA Cloning/Sequencing Workshop: A short course. Biochemistry and Biotechnology (Wiseman, A., Ed.), Ellis Horwood, Chichester, U.K.
Taylor, I., Patel, J., Firman, K. & Kneale, G. G. (1992). Purification and biochemical characterisation of the EcoR124 modification methylase. Nucleic Acids Research 20, 179-186.
Zinkevich, V., Heslop, P., Glover, S. W., Weiserova, M., Hubacek, J. & Firman, K. (1992). A mutation in the specificity polypeptide of the type I restriction endonuclease R.EcoK that affects subunit assembly. Journal of Molecular Biology 227, 597-601.
Patel, J., Taylor, I., Dutta, C. F., Kneale, G. G. & Firman, K. (1992). High-level expression of the cloned genes encoding the subunits of and the intact DNA methyltransferase, M.EcoR124. Gene 112, 21-27.
Abadjieva, A., Patel, J., Webb, M., Zinkevich, V. & Firman, K. (1993). A deletion mutant of the type IC restriction endonuclease EcoR124I expressing a novel DNA specificity. Nucleic Acids Research 21(19), 4435-4443.
Weiserova, M., Janscak, P., Benada, O., Hubacek, J., Zinkevich, V., Glover, S. W. & Firman, K. (1993). Cloning, production and characterisation of wild type and mutant forms of the R.EcoK endonucleases. Nucleic Acids Research 21(3), 373-379.
Abadjieva, A., Patel, J., Zinkevich, V. & Firman, K. (1994). Deletions within the DNA recognition subunit of M.EcoR124I that identify a region involved in protein-protein interactions between HsdS and HsdM. Journal of Molecular Biology 241(2), 35-43.
Hubacek, J., Weiserova, M., Janscak, P. & Firman, K. (1994). Restriction endonuclease R.EcoK and R.EcoR124 are probably located in different environments within the bacterial cell. Folia Microbiologica 39(2), 162-165.
Cherny, D. I., Kurakin, A. V., Lyamichev, V. I., Frank-Kamenetskii, M. D., Zinkevich, V. E., Firman, K. & Nielsenq, P. E. (1994). Electron microscopy of sequence-specific recognition of duplex by different ligands. J. Mol. Recogn. 7, 171-176.
Webb, M., Taylor, I. A., Firman, K. & Kneale, G. G. (1995). Probing the domain structure of the type IC DNA methyltransferase M.EcoR124I by limited proteolysis. Journal of Molecular Biology 280, 181-190.
Janscak, P., Abadjieva, A. & Firman, K. (1996). The type I restriction endonuclease R.EcoR124I: Over-production and biochemical properties. Journal of Molecular Biology 257, 977-991.
Szczelkun, M. D., Dillingham, M. S., Janscak, P., Firman, K. & Halford, S. E. (1996). Repercussions of DNA tracking by the type IC restriction endonuclease EcoR124I on linear, circular and catenated substrates. EMBO J. 15, 6335-6347.
Zinkevich, V., Popova, L., Kryukov, V., Abadjieva, A., Bogdarina, I., Janscak, P. & Firman, K. (1997). The HsdR subunit of R.EcoR124II: Cloning and over-expression of the gene and unexpected properties of the subunit. Nucleic Acids Res. 25, 503-510.
Szczelkun, M. D., Janscak, P., Firman, K. & Halford, S. E. (1997). Selection of non-specific DNA cleavage sites by the type IC restriction endonuclease EcoR124I. J. Mol. Biol. 271, 112-123.
Mernagh, D. R., Janscak, P., Firman, K. & Kneale, G. G. (1998). Protein-protein and protein-DNA interactions in the Type I restriction endonuclease R.EcoR124I. Biological Chemistry 379, 497-503.
Weiserova, M. & Firman, K. (1998). Isolation of a non-classical mutant of the DNA recognition subunit of the type I restriction endonuclease R.EcoR124I. Biological Chemistry 379, 585-589.
Janscák, P., Dryden, D. & Firman, K. (1998). Analysis of the subunit assembly of the type IC restriction-modification enzyme EcoR124I. Nucleic Acids Res. 26, 4439-4445.
Akimkina, T., Kostrov, S., Ivanov, P., Bonch-Osmolovskaya, L., Sokolova, T., Firman, K., Dutta, C. F. & McClellan, J. (1999). A Highly Conserved Plasmid from the Extreme Thermophile Themotoga maritima MC24 is Negatively Supercoiled. Plasmid 42, 236-240.
Janscák, P., Weiserová, M., Hubácek, J., Holubová, I., Dutta, C. F. & Firman, K. (2000). Two temperature-sensitive mutations in the DNA-binding subunit of EcoKI with differing properties. FEMS Microbiol. Lett. 182, 99-104.
Firman, K., Dutta, C., Weiserova, M. & Janscak, P. (2000). The role of subunit assembly in the functional control of type I restriction-modification enzymes. Molecular Biology Today 1, 1-8.
Holubová, I., Vejsadová, S., Weiserová, M. & Firman, K. (2000). Localisation of the type I restriction-modification enzyme EcoKI in the bacterial cell. Biochemical and Biophysical Research Communications 270, 46-51.
Firman, K. & Szczelkun, M. (2000). Measuring motion on DNA by the type I restriction endonuclease EcoR124I using triplex dissociation. European Molecular Biology Organisation Journal 19, 2094-2102.
Lisle, W., Dutta, C. F., Penner, M., Amitsur, M., Kaufmann, G. & Firman, K. (2000). Phage T4-encoded Stp alleviates the DNA restriction activity of EcoR124I endonuclease by affecting a critical step in the subunit assembly pathway. Mol. Biol. Today 1, 57-64.
Weiserova, M., Dutta, C. F. & Firman, K. (2000). A novel mutant of the type I restriction-modification enzyme EcoR124I is altered at a key stage in the subunit assembly pathway. J. Mol. Biol. 304, 301-310.
Roberts, R.J., et al., (2003) A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Research, 31, 1805-1812.
Abadjieva, A., Scarlett, G., Janscák, P., Dutta, C.F., and Firman, K. (2003) Characterisation of an EcoR124I DNA Restriction-Modification Enzyme Produced from a Deleted Form of the DNA-Binding Subunit, Which Results in a Novel DNA Specificity. Folia Microbiol. 48: 319-328.
Holubova, I., Stepánka, V., Firman, K. & Weiserova, M. (2004). Cellular Localization of Type I Restriction-Modification Enzymes is Family Dependent. Biochemical and Biophysical Research Communications 319, 375-380.
Firman, K. (2004) Enzymes as molecular motors. International Society for Optical Engineering Nanotechnology E-bulletin, June: 3-4.
Seidel, R., van Noort, J., van der Scheer, C., Bloom, J.G.P., Dekker, N.H., Dutta, C.F., Blundell, A., Robinson, T., Firman, K., and Dekker, C. (2004) Real-Time Observation of DNA Translocation by the Type I Restriction-Modification Enzyme EcoR124I. Nature Structural Biology 11: 838 - 843.
Pennadam, S.S., Firman, K., Alexander, C., and Górecki, D.C. (2004) Protein-polymer nano machines. Towards synthetic control of biological processes. Journal of Nanobiotechnology, 2: 1-7.
Pennadam, S., Lavigne, M. D., Dutta, C. F., Firman, K., Mernagh, D., Górecki, D. C. & Cameron, A. (2004). Control of A Multi-Subunit DNA Motor By A Thermo Responsive Polymer Switch. Journal of the American Chemical Society, 126(41): 13208-13209.
van Noort, J., van der Heijden, T., Dutta, C.F., Firman, K. and Dekker, C. (2004) Initiation of Translocation by Type I Restriction-Modification Enzymes is Associated with a Short DNA Extrusion. Nucleic Acids Res 32: 6540-6547.
Seidel, R., Bloom, J.G.P., van Noort, J., Dutta, C.F., Dekker, N.H., Firman, K., Szczelkun, M.D., and Dekker, C. (2005) Dynamics of initiation, termination and reinitiation of DNA translocation by the motor protein EcoR124I. European Molecular Biology Organisation Journal 24: 4188-4197.
Obarska, A., Blundell, A., Feder, M., Vejsadová, t., Šišáková, E., Weiserová, M., Bujnicki, J.M., and Firman, K. (2006) Structural Model for the Multisubunit Type IC Restriction-Modification DNA Methyltransferase M.EcoR124I in complex with DNA. Nucleic Acids Res 34: 1992-2005.
Youell, J. & K. Firman, (2007) Biological molecular motors for nanodevices. Nanotechnology Perceptions 3: 75-96.
Youell, J. & K. Firman, (2008) EcoR124I: from plasmid-encoded restriction modification system to nanodevice. Microbiology and Molecular Biology Reviews 72: 365–377.
Youell, J. & K. Firman, (2010) A single molecule nanoactuator: toward a device for drug discovery at the limits of sensitivity. In: International Beilstein Symposium on Functional Nanosciences. M. G. Hicks & C. Kettner (eds). Bozen, Italy: Logos-Verlag, pp. 73-92.
Youell, J., Fordham, D. and Firman, K. (2011). Production and single-step purification of EGFP and a biotinylated version of the Human Rhinovirus 14 3C protease. Protein Expression and Purification 79, 258-262.
Youell, J. and Firman, K. (2012). Title Mechanistic insight into Type I restriction endonucleases Frontiers In Bioscience 17, 2122-2139.
Last modified on
23 December 2011
© Dr Keith Firman
Author Dr Keith Firman.