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It has been known for some time that addition of a non-hydrolysable analogue of ATP (e.g. ATPgS) not only prevents translocation of the DNA, but also 'freezes' the initiation complex. Therefore,
van Noort et al (2004) used ATPgS
to trap initial translocation complexes, which were visualized
by Atomic Force Microscopy (AFM). In the R1-complex,
a small bulge was observed using atomic force microscopy (AFM),
that is associated with a shortening in the contour-length of
the DNA by 8 nm. This bulge was found to be sensitive to
single-strand |
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Portsmouth |