Restriction-Modification Systems
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Introduction to site:This Internet Site describes Type I Restriction-Modification Systems. These enzyme were the first discovered of all of the restriction enzymes and pioneered studies of restriction endonucleases, which eventually led to the discovery of Type II Restriction-Modification, their characterisation as site-specific endonucleases and the development of Genetic Engineering. Type I R-M enzymes are NOT site-specific endonucleases and have not found a use in Genetic Engineering, which means they have been somewhat neglected over the years. However, they a very interesting multisubunit, multifunctional molecular motors with strong relationships to both recombination enzymes such as RecBC and to chromatin remodeling factors in eukaryotes. Recently, there has been a resurgence of interest in these enzymes, in part spurred by investigations that have addressed some of the early questions first raised by the presence of Restriction-Modification systems (Murray, 2000), but more recently due to the single-molecule studies of the enzymes as molecular motors (Seidel et al., 2004) These web pages are a resource primarily aimed at those involved in research in this area, but will also provide useful background information to undergraduates and graduate students.
Contact Information:You can contact the Web Team and Dr Keith Firman using the details below (please explain you are contacting about the Type I R-M Web pages):
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